Comparison of Two Direct Assays for Platelet-Associated IgG (PAIgG) in Assessment of Immune and Nonimmune Thrombocytopenia

A number of methods have been developed to measure platelet-associated lgG (PAIgG). The antiglobulin consumption assay directly quantitates lgG on the platelet and is sensitive and specific. A fluorescent anti-lgG assay has recently been described and has the advantage of simplicity. We compared the results of these two PAIgG assays in immune and nonimmune thrombocytopenia and nonthrombocytopenic controls. The antiglobulin consumption assay was negative in 61 of 62 and the fluorescent negative in 54 of 62 assays in nonthrombocytopenic controls. and they were negative in 1 1 of 13 and 8 of 1 3 assays, respectively, in nonimmune thrombocytopenic patients. The antiglobulin consumption assay was positive in 54 of 58 and the fluorescent positive in 24 of 58 assays of patients with immune thrombocytopenia (ITP and SLE). The overall sensitivity and specificity of the antiglobulin consumption assay was 94% and 95% and of the fluorescent assay was 44% and 82%.